Phenotypic instability of Saos-2 cells in long-term culture.
Biochem Biophys Res Commun
; 333(1): 216-22, 2005 Jul 22.
Article
en En
| MEDLINE
| ID: mdl-15939397
ABSTRACT
The human osteosarcoma cell line Saos-2 is widely used as a model system for human osteoblastic cells, though its phenotypic stability has not been ascertained. We therefore propagated these cells over 100 passages and compared relevant phenotypic properties. In general, higher passage cells exhibited higher proliferation rates and lower specific alkaline phosphatase activities, though mineralization was significantly more pronounced in cultures of late passage cells. Whereas expression of most genes investigated did not vary profoundly, some genes exhibited remarkable differences. Decorin, for instance, that has been discussed as a regulator of proliferation and mineralization, is strongly expressed only in early passage cells, and two receptors for pleiotrophin and midkine exhibited an almost mutually exclusive expression pattern in early and late passage cells, respectively. Our observations indicate that special care is required when results obtained with Saos-2 cells with different culture history are to be compared.
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Bases de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Fenotipo
/
Osteosarcoma
/
Técnicas de Cultivo de Célula
/
Inestabilidad Genómica
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2005
Tipo del documento:
Article
País de afiliación:
Alemania