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The RNA-binding protein fragile X-related 1 regulates somite formation in Xenopus laevis.
Huot, Marc-Etienne; Bisson, Nicolas; Davidovic, Laetitia; Mazroui, Rachid; Labelle, Yves; Moss, Tom; Khandjian, Edouard W.
Afiliación
  • Huot ME; Unité de recherche en génétique humaine et moléculaire, CHUQ-St-François d'Assise, Québec, Québec G1L 3L5, Canada.
Mol Biol Cell ; 16(9): 4350-61, 2005 Sep.
Article en En | MEDLINE | ID: mdl-16000371
Fragile X-related 1 protein (FXR1P) is a member of a small family of RNA-binding proteins that includes the Fragile X mental retardation 1 protein (FMR1P) and the Fragile X-related 2 protein (FXR2P). These proteins are thought to transport mRNA and to control their translation. While FMR1P is highly expressed in neurons, substantial levels of FXR1P are found in striated muscles and heart, which are devoid of FMRP and FXR2P. However, little is known about the functions of FXR1P. We have isolated cDNAs for Xenopus Fxr1 and found that two specific splice variants are conserved in evolution. Knockdown of xFxr1p in Xenopus had highly muscle-specific effects, normal MyoD expression being disrupted, somitic myotomal cell rotation and segmentation being inhibited, and dermatome formation being abnormal. Consistent with the absence of the long muscle-specific xFxr1p isoform during early somite formation, these effects could be rescued by both the long and short mRNA variants. Microarray analyses showed that xFxr1p depletion affected the expression of 129 known genes of which 50% were implicated in muscle and nervous system formation. These studies shed significant new light on Fxr1p function(s).
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas de Unión al ARN / Somitos / Proteínas de Xenopus / Proteína de la Discapacidad Intelectual del Síndrome del Cromosoma X Frágil Límite: Animals / Humans Idioma: En Revista: Mol Biol Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2005 Tipo del documento: Article País de afiliación: Canadá

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas de Unión al ARN / Somitos / Proteínas de Xenopus / Proteína de la Discapacidad Intelectual del Síndrome del Cromosoma X Frágil Límite: Animals / Humans Idioma: En Revista: Mol Biol Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2005 Tipo del documento: Article País de afiliación: Canadá