Cytokine-induced stable neuronal differentiation of human bone marrow mesenchymal stem cells in a serum/feeder cell-free condition.
Dev Growth Differ
; 47(6): 423-33, 2005 Aug.
Article
en En
| MEDLINE
| ID: mdl-16109040
ABSTRACT
The characteristics and multilineage differentiation potential of bone marrow mesenchymal stem cells (BM MSC) remain controversial. This study aimed to characterize human BM MSC isolated by plastic adherent or antibody selection and their neuronal differentiation potential using growth factors or chemical inducing agents. MSC were found to express low levels of neuronal markers neurofilament-M, beta tubulin III, and neuron specific enolase. Under a serum- and feeder cell-free condition, basic fibroblast growth factor, epidermal growth factor, and platelet-derived growth factor induced neuronal morphology in MSC. In addition to the above markers, these cells expressed neurotransmitters or associated proteins gamma-aminobutyric acid, tyrosine hydroxylase and serotonin. These changes were maintained for up to 3 months in all bone marrow specimens (N = 6). In contrast, butylated hydroxyanisole and dimethylsulfoxide were unable to induce sustained neuronal differentiation. Our results show that MSC isolated by two different procedures produced identical lineage differentiation with defined growth factors in a serum- and feeder cell-free condition.
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Bases de datos:
MEDLINE
Asunto principal:
Células de la Médula Ósea
/
Citocinas
/
Células Madre Mesenquimatosas
/
Neuronas
Límite:
Humans
Idioma:
En
Revista:
Dev Growth Differ
Año:
2005
Tipo del documento:
Article
País de afiliación:
Australia