[PCR detection of transgenic elements in feed raw material].
Yi Chuan
; 24(3): 293-6, 2002 May.
Article
en Zh
| MEDLINE
| ID: mdl-16126685
ABSTRACT
Based on the heterogeneous genes usually used in transgenic crops, the PCR technique was performed with primers derived from CaMV 35S promoter (35S-promoter,originated from cauliflower mosaic virus), NOS terminator (nopaline synthase-terminator,derived from Agrobacterium tumefaciens), EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene, and CryIA(b) (delta-endotoxin,evolved from Bacillus thuringiensis subsp. kurstaki) gene to detect transgenic agents from feed raw materials of soybean dregs and corn gluten meal, respectively. Endogenous corn Zein (a protein extracted from corn gluten) gene, soybean Lectin (chitin-binding protein) gene and negative, positive control were applied for avoiding false results. The method established here has been successfully applied in detecting transgenic elements in imported feed raw material.
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Bases de datos:
MEDLINE
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Yi Chuan
Asunto de la revista:
GENETICA
Año:
2002
Tipo del documento:
Article
País de afiliación:
China