High-throughput cultivation of microorganisms using microcapsules.
Methods Enzymol
; 397: 124-30, 2005.
Article
en En
| MEDLINE
| ID: mdl-16260288
ABSTRACT
This chapter describes a universal and novel method that provides access to the immense reservoir of untapped microbial diversity by cultivation. This technique uses microcapsules to encapsulate single cells combined with parallel microbial cultivation under low nutrient flux conditions. Under these conditions, single encapsulated cells grow and form microcolonies within the microcapsules. Flow cytometry is used as a sensitive tool to detect growth within the microcapsules. Microcapsules that contain microcolonies (originated from a single encapsulated cell) are sorted individually into microtiter dishes containing organic-rich medium. This high-throughput cultivation can provide more than 10,000 bacterial and fungal isolates per environmental sample.
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Bases de datos:
MEDLINE
Asunto principal:
Bacterias Anaerobias
/
Cápsulas
/
Técnicas Bacteriológicas
Idioma:
En
Revista:
Methods Enzymol
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos