Platelet ADP response deteriorates in synthetic storage media.
Transfusion
; 46(2): 204-12, 2006 Feb.
Article
en En
| MEDLINE
| ID: mdl-16441596
ABSTRACT
BACKGROUND:
During storage under blood bank conditions, platelets (PLTs) are known to secrete ADP. PLT stimulation by ADP results in refractoriness to restimulation, making this response one of the most unstable PLT reactions. The goal of this study was to evaluate the ADP-induced responses of PLTs stored in full plasma or in plasma and additive solution (AS). STUDY DESIGN ANDMETHODS:
Surface expression of P-selectin, ADP-induced aggregation, and reconstituted whole-blood thrombus formation were determined on collagen surfaces in a perfusion model with PLTs that were stored for 4 days either in plasma or in the presence of plasma with PAS-II or Composol.RESULTS:
After 4 days of storage in PAS-II but not in Composol, the percentage of PLTs that had secreted granule contents (P-selectin) was increased, when compared to PLTs stored in full plasma. Maximal aggregation in response to ADP was reduced for PLTs stored in PAS-II or Composol. Resuspension of these PLTs in plasma at 37 degrees C for 1 hour caused partial recovery of the aggregation response. Addition of apyrase to PLTs in AS preserved the responsiveness toward ADP. Titration experiments indicated that this response gradually decreased with decreasing plasma concentration. The functional significance of these findings was demonstrated by perfusion experiments. Thrombus formation on collagen was significantly higher for PLTs stored in full plasma than for PLTs stored in PAS-II or Composol.CONCLUSIONS:
Storage of PLTs in the presence of AS under blood bank conditions induces deterioration of the PLT responsiveness to ADP compared to PLT concentrates in 100 percent plasma. Higher plasma-to-AS ratios result in better preserved responses.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Plaquetas
/
Conservación de la Sangre
/
Adenosina Difosfato
/
Soluciones Preservantes de Órganos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Transfusion
Año:
2006
Tipo del documento:
Article
País de afiliación:
Países Bajos