Responses of rat pulp cells to heat stress in vitro.
J Dent Res
; 85(5): 432-5, 2006 May.
Article
en En
| MEDLINE
| ID: mdl-16632756
ABSTRACT
Although heat stress can cause irritation in the dentin/pulp complex, little is known about the thermotolerance of pulp cells and their response to heat stress. We investigated cultured rat pulp cell responses to heat stress. Cells were subjected to a temperature of 42 degrees C for 30 minutes, and HSPs, alkaline phosphatase activity, and gap-junctional communication were determined at various time points. Although only low levels of HSP70 expression were detected before heat treatment, heat shock markedly induced HSP70 expression, with it gradually increasing at 1 hour after being heated. HSP25, however, showed no dramatic change. Gap junction protein connexin43 rapidly degraded after heat treatment, recovering to normal levels within the following 6 hours. Alkaline phosphatase activity decreased immediately after heat stress, recovering after 1 hour. These results indicate that dental pulp possesses protective factors, including HSPs, and that it can recover viability of intercellular communication and alkaline phosphatase activity after heat stress.
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Bases de datos:
MEDLINE
Asunto principal:
Respuesta al Choque Térmico
/
Trastornos de Estrés por Calor
/
Pulpa Dental
Límite:
Animals
Idioma:
En
Revista:
J Dent Res
Año:
2006
Tipo del documento:
Article
País de afiliación:
Japón