Microinjection of targeted embryonic stem cells and establishment of knockout mouse lines for Fmo genes.
Methods Mol Biol
; 320: 329-41, 2006.
Article
en En
| MEDLINE
| ID: mdl-16719404
ABSTRACT
Methods are described for the injection of mouse embryonic stem cells, in which Fmo genes have been targeted to disrupt gene function, into 3.5-d-old blastocysts and the implantation of these into foster mothers. Successful injection and implantation of blastocysts will produce mice of mixed coat color (the chimera). Also described are methods to establish the success of blastocyst injection and implantation of germ-line transmission of the knockout (KO) mutation. Breeding strategies to produce congenic and isogenic KO mouse lines are outlined. Simple methods for the isolation of tail DNA, the tagging of mice, and record keeping of the line are also given.
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Bases de datos:
MEDLINE
Asunto principal:
Oxigenasas
/
Células Madre
/
Embrión de Mamíferos
Tipo de estudio:
Guideline
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2006
Tipo del documento:
Article
País de afiliación:
Reino Unido