Genetically engineered pfabA pfabR bacteria: an electrochemical whole cell biosensor for detection of water toxicity.
Anal Chem
; 78(14): 4952-6, 2006 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-16841916
ABSTRACT
We describe here a bacterial sensor for electrochemical detection of toxic chemicals. The sensor constitutes recombinant bacteria harboring plasmids encoding the fabA and fabR genes and has high-resolution amperometric response to membrane-damaging chemicals. For example, it can detect phenol at concentrations ranging between 1.6 and 16 ppm within 20 min. The high sensitivity is achieved by using the fabA promoter fused to a reporter gene-encoded beta-galactosidase on a low copy number plasmid, under the control of the FabR repressor. The use of electrochemical whole cell sensors enables sensitive, fast, easy to operate, and cost-effective detection of water toxicity threats.
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Bases de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Contaminantes Químicos del Agua
/
Técnicas Biosensibles
/
Hidroliasas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Chem
Año:
2006
Tipo del documento:
Article