Human serum for tissue engineering of human nasal septal cartilage.
Otolaryngol Head Neck Surg
; 135(3): 397-403, 2006 Sep.
Article
en En
| MEDLINE
| ID: mdl-16949971
ABSTRACT
OBJECTIVE:
To compare the chondrogenic and proliferative effects of pooled human serum (HS) and fetal bovine serum (FBS) on tissue-engineered human nasal septal chondrocytes. STUDY DESIGN ANDSETTING:
Human chondrocytes were expanded for one passage in monolayer in medium supplemented with 10% FBS, 2% HS, 10% HS, or 20% HS. Cells were then suspended in alginate beads for 3D culture for 2 weeks with 10% FBS, 2% HS, 10% HS, or 20% HS.RESULTS:
Monolayer cell yields were greater with HS than FBS. In alginate, cellular proliferation, glycosaminoglycan production per cell, and type II collagen were significantly higher with 10% HS compared to 10% FBS controls.CONCLUSION:
HS results in increased proliferation and production of cartilaginous extracellular matrix by tissue-engineered human nasal septal chondrocytes, compared to FBS controls.SIGNIFICANCE:
Culture with human serum may facilitate creation of neocartilage constructs that more closely resemble native tissue.
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Bases de datos:
MEDLINE
Asunto principal:
Sangre
/
Ingeniería de Tejidos
/
Tabique Nasal
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Otolaryngol Head Neck Surg
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos