Aptamer-capped nanocrystal quantum dots: a new method for label-free protein detection.
J Am Chem Soc
; 128(49): 15584-5, 2006 Dec 13.
Article
en En
| MEDLINE
| ID: mdl-17147356
ABSTRACT
We demonstrate that aptamer-capped near-infrared PbS quantum dots (QDs) can detect a target protein based on selective charge transfer. The water-soluble QDs are synthesized with the thrombin-binding aptamer, which retains the secondary quadruplex structure necessary for binding to thrombin. These QDs have diameters of 3-6 nm and fluoresce around 1050 nm. When the aptamer-functionalized QD binds to its target, a fluorescence quenching occurs due to charge transfer from amine groups on the protein to the QD. Thrombin is detected within 1 min with a detection limit of approximately 1 nM. This selective detection is observed even in the presence of high background concentrations of interfering negatively or positively charged proteins, suggesting that aptamer-capped QDs could be useful for label-free protein assays.
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Bases de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Proteínas
/
Puntos Cuánticos
/
Aptámeros de Nucleótidos
/
Nanopartículas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Am Chem Soc
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos