Two HlyIIR dimers bind to a long perfect inverted repeat in the operator of the hemolysin II gene from Bacillus cereus.
FEBS Lett
; 581(6): 1190-6, 2007 Mar 20.
Article
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| MEDLINE
| ID: mdl-17346714
ABSTRACT
HlyIIR is a negative transcriptional regulator of hemolysin II gene from B. cereus. It binds to a long DNA perfect inverted repeat (44bp) located upstream the hlyII gene. Here we show that HlyIIR is dimeric in solution and in bacterial cells. No protein-protein interactions between dimers and no significant modification of target DNA conformation upon complex formation were observed. Two HlyIIR dimers were found to bind to native operator independently with Kd level in the nanomolar range. The minimal HlyIIR binding site was identified as a half of the long DNA perfect inverted repeat.
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Bases de datos:
MEDLINE
Asunto principal:
Bacillus cereus
/
Proteínas Bacterianas
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Toxinas Bacterianas
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Secuencias Repetitivas de Ácidos Nucleicos
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Regiones Operadoras Genéticas
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Proteínas Hemolisinas
Idioma:
En
Revista:
FEBS Lett
Año:
2007
Tipo del documento:
Article
País de afiliación:
Rusia