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The TKTL1 gene influences total transketolase activity and cell proliferation in human colon cancer LoVo cells.
Hu, Li-Hua; Yang, Ju-Hong; Zhang, De-Tai; Zhang, Song; Wang, Lin; Cai, Peng-Cheng; Zheng, Jian-Feng; Huang, Jin-Song.
Afiliación
  • Hu LH; Department of Laboratory, Union Hospital of Tongji Medical College, Huazhong University of Science & Technology, Wuhan, PRC. hulihuajy@yahoo.com.cn
Anticancer Drugs ; 18(4): 427-33, 2007 Apr.
Article en En | MEDLINE | ID: mdl-17351395
A plasmid carrying DNA to be transcribed into a small interfering RNA against transketolase-like-1 mRNA was constructed and transfected into a human colon cancer cell line. The mRNA expression of transketolase gene family in the human colon cell line was determined by real-time polymerase chain reaction. The effect of anti-transketolase-like-1 small interfering RNA on cell proliferation and cell cycle in the human colon cancer cell line cells was detected by flow cytometry and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide. The transketolase-like-1 gene was significantly downregulated in human colon cancer cell line cells transfected with small interfering RNA transketolase-like-1 constructs compared with the cells transfected with control vector and the cells without transfection. In addition, the anti-transketolase-like-1 small interfering RNA construct significantly decreased the level of transketolase in the transfected human colon cancer cell line cells, arrested them in G0/G1 phase and substantially inhibited cell proliferation. No significant difference was found in the other two genes (transketolase and transketolase-like-2 genes) between the transfected human colon cancer cell line cells and the controls (P>0.05). Our data demonstrated that the transketolase-like-1 gene plays an important role in total transketolase activity and in the cell proliferation of human colon cancer. Transketolase-like-1 may serve as a target for novel anticancer therapies.
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Bases de datos: MEDLINE Asunto principal: Transcetolasa / Neoplasias del Colon Límite: Humans Idioma: En Revista: Anticancer Drugs Asunto de la revista: ANTINEOPLASICOS Año: 2007 Tipo del documento: Article
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Bases de datos: MEDLINE Asunto principal: Transcetolasa / Neoplasias del Colon Límite: Humans Idioma: En Revista: Anticancer Drugs Asunto de la revista: ANTINEOPLASICOS Año: 2007 Tipo del documento: Article