[Study on the effect of electric field on the secondary structure of lipase by circular dichroism].
Guang Pu Xue Yu Guang Pu Fen Xi
; 26(12): 2311-4, 2006 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-17361738
ABSTRACT
After the lipase was treated with electric field for five minutes, the effect of electric field on the secondary structure of lipase was studied by circular dichroism (CD). The results showed that different electric field strength in the range from 0. 5 to 6. 0 kV x cm(-1) has a different effect on the relative contents of alpha-helix, beta-sheet, beta-turn and random coil of the lipase, and whose changes were non-monotonous with the raising of electric field. In general, the electric treatment tends to transform the alpha-helix and beta-sheet into beta-turn and random coil. The decrease in alpha-helix and beta-sheet ranged respectively from 4. 6% to 48. 0% and from 13. 2% to 35. 1%, and the increase in beta-turn and random coil ranged respectively from 2. 8% to 33. 3% and from 0. 9% to 48. 1%. The result of this study has important meaning to explain the biological effect of electric treatment seeds.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Dicroismo Circular
/
Lipasa
Idioma:
Zh
Revista:
Guang Pu Xue Yu Guang Pu Fen Xi
Año:
2006
Tipo del documento:
Article
País de afiliación:
China