Production of monoclonal antibodies reactive with a denatured form of the Friend murine leukemia virus gp70 envelope protein: use in a focal infectivity assay, immunohistochemical studies, electron microscopy and western blotting.
J Virol Methods
; 34(3): 255-71, 1991 Oct.
Article
en En
| MEDLINE
| ID: mdl-1744218
ABSTRACT
Four monoclonal antibodies were selected for their ability to recognize the envelope protein of Friend murine leukemia virus (F-MuLV) in methanol-fixed tissue culture cells. Each of these monoclonal antibodies was found to react only with F-MuLV. By using recombinant retroviruses, it was determined that each of the monoclonal antibodies recognized the C-terminal one-third of the F-MuLV gp70 envelope protein. The monoclonal antibodies were effective in radioimmunoprecipitation of F-MuLV proteins, and one of the antibodies, 720, was also effective in Western blotting. The ability of antibody 720 to react with F-MuLV in methanol-fixed cells facilitated the use of a sensitive immunoperoxidase method with a focal virus infectivity assay. In immunohistochemical studies using light microscopy, antibody 720 could specifically label F-MuLV-infected cells in acetone-fixed tissue sections from F-MuLV-infected animals. Finally, in immuno-gold labelling studies using electron microscopy, antibody 720 could be used to distinguish F-MuLV from amphotropic MuLV.
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Bases de datos:
MEDLINE
Asunto principal:
Proteínas del Envoltorio Viral
/
Virus de la Leucemia Murina de Friend
/
Anticuerpos Monoclonales
/
Anticuerpos Antivirales
Límite:
Animals
Idioma:
En
Revista:
J Virol Methods
Año:
1991
Tipo del documento:
Article