Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes.
Genome Biol
; 8(5): R87, 2007.
Article
en En
| MEDLINE
| ID: mdl-17519022
ABSTRACT
We have developed a new microarray-based genetic technique, named MGK (Monitoring of Gene Knockouts), for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids, and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics studies.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Bacterias
/
Silenciador del Gen
/
Genes Bacterianos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Genome Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos