The Golgi-associated protein GRASP is required for unconventional protein secretion during development.
Cell
; 130(3): 524-34, 2007 Aug 10.
Article
en En
| MEDLINE
| ID: mdl-17655921
During Dictyostelium development, prespore cells secrete acyl-CoA binding protein (AcbA). Upon release, AcbA is processed to generate a peptide called spore differentiation factor-2 (SDF-2), which triggers terminal differentiation of spore cells. We have found that cells lacking Golgi reassembly stacking protein (GRASP), a protein attached peripherally to the cytoplasmic surface of Golgi membranes, fail to secrete AcbA and, thus, produce inviable spores. Surprisingly, AcbA lacks a signal sequence and is not secreted via the conventional secretory pathway (endoplasmic reticulum-Golgi-cell surface). GRASP is not required for conventional protein secretion, growth, and the viability of vegetative cells. Our findings reveal a physiological role of GRASP and provide a means to understand unconventional secretion and its role in development.
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Bases de datos:
MEDLINE
Asunto principal:
Proteínas Protozoarias
/
Adenilil Ciclasas
/
Dictyostelium
/
Aparato de Golgi
/
Proteínas de la Membrana
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Cell
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos