Analysis of the serological variability of Lettuce mosaic virus using monoclonal antibodies and surface plasmon resonance technology.
J Gen Virol
; 88(Pt 9): 2605-2610, 2007 Sep.
Article
en En
| MEDLINE
| ID: mdl-17698673
ABSTRACT
A panel of 19 monoclonal antibodies (mAbs) was used to study the immunological variability of Lettuce mosaic virus (LMV), a member of the genus Potyvirus, and to perform a first epitope characterization of this virus. Based on their specificity of recognition against a panel of 15 LMV isolates, the mAbs could be clustered in seven reactivity groups. Surface plasmon resonance analysis indicated the presence, on the LMV particles, of at least five independent recognition/binding regions, correlating with the seven mAbs reactivity groups. The results demonstrate that LMV shows significant serological variability and shed light on the LMV epitope structure. The various mAbs should prove a new and efficient tool for LMV diagnostic and field epidemiology studies.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Virus de Plantas
/
Variación Genética
/
Lactuca
/
Anticuerpos Monoclonales
/
Virus del Mosaico
Idioma:
En
Revista:
J Gen Virol
Año:
2007
Tipo del documento:
Article
País de afiliación:
Francia