Bacterial surface display of GFP(uv) on bacillus subtilis spores.
J Microbiol Biotechnol
; 17(4): 677-80, 2007 Apr.
Article
en En
| MEDLINE
| ID: mdl-18051283
ABSTRACT
To analyze a cotG-based Bacillus subtilis spore display system directly, GFP(uv) was expressed on the surface of Bacillus subtilis spores. When GFP(uv) was fused to the C-terminal of the cotG structural gene and expressed, the existence of a CotG-GFP(uv) fusion protein on the B. subtilis spore was confirmed by flow cytometry confocal microscopic analysis. When the cotG anchoring motif was deleted, no fluorescence emission was observed under flow cytometry and confocal microscopic analysis from the purified spore, confirming the essential role of CotG as an anchoring motif. This GFP(uv) displaying spore might be used for another signaling application triggered by intracellular or extracellular stimuli.
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Bases de datos:
MEDLINE
Asunto principal:
Bacillus subtilis
/
Proteínas Bacterianas
/
Proteínas Recombinantes de Fusión
/
Proteínas Fluorescentes Verdes
Idioma:
En
Revista:
J Microbiol Biotechnol
Año:
2007
Tipo del documento:
Article