Protein production and purification.
Nat Methods
; 5(2): 135-46, 2008 Feb.
Article
en En
| MEDLINE
| ID: mdl-18235434
ABSTRACT
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes
/
Ingeniería de Proteínas
/
Química Física
/
Proteómica
/
Fraccionamiento Químico
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2008
Tipo del documento:
Article