[Isolation of multipotent mesenchymal stem cells from the tissue of umbilical cord for osteoblasts and adipocytes differentiation].

ABSTRACT

OBJECTIVE: To explore the methods and conditions for isolating and proliferating multipotent mesenchymal stem cells (MSCs) from the tissue of umbilical cord, with an aim to induce osteogenic and adipogenic differentiation in vitro. METHOD: The cord was dissected along the long axis, with vessels pulled away and then sutured into a "loop". Collagenase solution was filled into the loop. Suspended cells were collected from the loop suspension after 6-8 hours and centrifuged. The cells were finally cultured in polystyrene dishes. The single cell-derived colonies were obtained and tested for their immunophenotype and osteoblast and lipoblast differentiations. RESULT: Adherent cells were obtained from the tissue of umbilical cord, which proliferated and formed single cell-derived colonies. The colonies presented matrix cells immunophenotype and differentiated into osteoblasts that produced mineralized matrices, which were stained by alizarin red and alkaline phosphatase. The colonies also differentiated into adipocytes that accumulated lipid vacuoles, which were demonstrated by the morphology and oil red stains. CONCLUSION: MSCs can be isolate from the tissue of umbilical cords and proliferate in vitro. The proliferated colonies show matrix cell immunophenotypes and can differentiate into osteoblasts and adipocytes.