Claviceps nigricans and Claviceps grohii: their alkaloids and phylogenetic placement.
J Nat Prod
; 71(6): 1085-8, 2008 Jun.
Article
en En
| MEDLINE
| ID: mdl-18461998
ABSTRACT
Claviceps purpurea, C. grohii, C. zizaniae, C. cyperi, and C. nigricans are closely related ergot fungi and form a monophyletic clade inside the genus Claviceps. Analysis of alkaloid content in C. nigricans sclerotia using UPLC detected ergocristine (1), ergosine (2), alpha-ergocryptine (3), and ergocristam (4). Alkaloids 1, 3, and 4 were found in the sclerotia of C. grohii. The content of 4 in the mixture of alkaloids from C. nigricans and C. grohii (over 8% and over 20%, respectively) was unusually high. Submerged shaken cultures of C. nigricans produced no alkaloids, whereas C. grohii culture formed small amounts (15 mg L (-1)) of extracellular clavines and 1. In the previously used HPLC method the ergocristam degradation product could have been obscured by the ergosine peak. Therefore sclerotia of a C. purpurea habitat-specific population G2 with the dominant production of 1 and 2 have been reanalyzed, but no 4 was detected. The phylogeny of the C. purpurea-related species group is discussed with regard to alkaloid-specific nonribosomal peptide synthetase duplication leading to the production of two main ergopeptines instead of a single product.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Claviceps
/
Alcaloides
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Nat Prod
Año:
2008
Tipo del documento:
Article
País de afiliación:
República Checa