Differential detection of phosphorylated glycogen synthase kinase 3 alpha and beta depending on blocking conditions.
Anal Biochem
; 379(1): 136-7, 2008 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-18485882
ABSTRACT
It is generally recommended that immunoblots using phosphospecific antibodies be performed using bovine serum albumin (BSA) instead of milk. There are two subtypes of glycogen synthase kinase 3 (GSK3), GSK3 alpha and GSK3 beta, with apparent molecular weights of 51 and 49 kDa, respectively. Here we show that immunoblots performed using 5% milk allow the detection of both phosphorylated GSK3 alpha and phosphorylated GSK3 beta, whereas only phosphorylated GSK3 alpha is visible when immunoblots are performed using 3% BSA.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Immunoblotting
/
Glucógeno Sintasa Quinasa 3
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Biochem
Año:
2008
Tipo del documento:
Article
País de afiliación:
Reino Unido