Direct antimicrobial susceptibility testing method for analysis of sputum collected from patients with cystic fibrosis.
J Cyst Fibros
; 7(3): 238-43, 2008 May.
Article
en En
| MEDLINE
| ID: mdl-18498727
ABSTRACT
BACKGROUND:
Chronic Pseudomonas aeruginosa colonisation and subsequent exacerbations in patients with cystic fibrosis (CF) require antimicrobial treatment. But since multiple morphotypes and other Gram-negative bacteria with different antibiotic susceptibilities are often isolated inside the same sputum sample, bacteriological analysis is difficult.METHODS:
To simplify this analysis, we explored a direct sputum antimicrobial susceptibility testing (DSST) method by applying E test directly on plates inoculated with the sputum. A total of 316 samples collected from CF patients were analysed and compared with standard procedures (SP) for the identification and antimicrobial susceptibility testing of all Gram-negative bacterial species.RESULTS:
DSST was as efficient as SP to detect P. aeruginosa including the mucoid morphotype in monomicrobial specimen, but was less sensible to detect all Gram-negative bacteria present in the same sample. It allowed the direct reading of the MIC inhibiting all Gram-negative bacteria. Agreements between these global MICs with the cumulative antibiotics susceptibility of all Gram-negative bacteria measured by SP were excellent for tobramycin and imipenem (>96%) and satisfactory for ticarcillin, ceftazidime, aztreonam and ciprofloxacin (90.4% to 94.3%). In conclusion, the DSST method is an efficient and easy antibiotic susceptibility testing method.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Esputo
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Pruebas de Sensibilidad Microbiana
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Fibrosis Quística
Tipo de estudio:
Clinical_trials
Límite:
Adolescent
/
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
J Cyst Fibros
Año:
2008
Tipo del documento:
Article
País de afiliación:
Francia