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Immunological studies on the rat peripheral-type benzodiazepine acceptor.
Moynagh, P N; Bailey, C J; Boyce, S J; Williams, D C.
Afiliación
  • Moynagh PN; Department of Biochemistry, University of Dublin, Trinity College, Ireland.
Biochem J ; 275 ( Pt 2): 419-25, 1991 Apr 15.
Article en En | MEDLINE | ID: mdl-1850987
Photoaffinity labelling of rat adrenal mitochondrial preparations with [3H]PK 14105 resulted in a single 3H-labelled band on SDS/PAGE gels with an apparent-molecular-mass peak of 18 kDa. This represents a polypeptide associated with the peripheral-type benzodiazepine-binding site. Solubilization of photoaffinity-labelled membranes with 6 M-guanidine hydrochloride, followed by gel filtration and reversed-phase h.p.l.c. of the solubilized material, resulted in the purification to homogeneity of the [3H]PK 14105-labelled polypeptide. This purified polypeptide was used to raise a rabbit polyclonal antiserum which recognized the immunogen in pure form and exclusively recognized it in a crude preparation of rat adrenal mitochondria as judged by immunoblotting. By the same analysis the antiserum identified the corresponding polypeptide from rat kidney and salivary gland, demonstrating its cross-reactivity. Subsequent immunocytochemical studies localized the polypeptide to the cortex of the adrenal gland, the distal tubules of kidney, the interstitial cells of testis, the biliary epithelium of liver and the choroid plexus and ependyma cells within the brain. This selective localization within organs may provide an insight into the physiological role of the peripheral-type benzodiazepine acceptor.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Glándulas Suprarrenales / Receptores de GABA-A / Isoquinolinas / Riñón / Mitocondrias Límite: Animals Idioma: En Revista: Biochem J Año: 1991 Tipo del documento: Article País de afiliación: Irlanda

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Glándulas Suprarrenales / Receptores de GABA-A / Isoquinolinas / Riñón / Mitocondrias Límite: Animals Idioma: En Revista: Biochem J Año: 1991 Tipo del documento: Article País de afiliación: Irlanda