A single amino acid substitution in one of the lipases of Aspergillus nidulans confers resistance to the antimycotic drug undecanoic acid.
Biochem Genet
; 46(9-10): 557-65, 2008 Oct.
Article
en En
| MEDLINE
| ID: mdl-18516670
ABSTRACT
A plausible approach to evaluate the inhibitory action of antifungals is through the investigation of the fungal resistance to these drugs. We describe here the molecular cloning and initial characterization of the A. nidulans lipA gene, where mutation (lipA1) conferred resistance to undecanoic acid, the most fungitoxic fatty acid in the C(70)-C(180) series. The lipA gene codes for a putative lipase with the sequence consensus GVSIS and WIFGGG as the catalytic signature. Comparison of the wild-type and LIP1 mutant strain nucleotide sequences showed a G --> A change in lipA1 allele, which results in a Glu(214) --> Lys substitution in LipA protein. This ionic charge change in a conserved LipA region, next to its catalytic site, may have altered the catalytic properties of this enzyme resulting in resistance to undecanoic acid.
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1
Bases de datos:
MEDLINE
Asunto principal:
Aspergillus nidulans
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Hidrolasas de Éster Carboxílico
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Farmacorresistencia Fúngica
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Ácidos Grasos
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Aminoácidos
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Lipasa
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Antifúngicos
Idioma:
En
Revista:
Biochem Genet
Año:
2008
Tipo del documento:
Article
País de afiliación:
Brasil