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Tx3-4 a toxin from the venom of spider Phoneutria nigriventer blocks calcium channels associated with exocytosis.
de Castro Junior, Célio José; Pinheiro, Ana Cristina N; Guatimosim, Cristina; Cordeiro, Marta N; Souza, Alessandra H; Richardson, Michael; Romano-Silva, Marco A; Prado, Marco Antônio M; Gomez, Marcus V.
Afiliación
  • de Castro Junior CJ; Departamento Farmacologia BeloHorizonte, MG, Brazil. celiojcjunior@yahoo.com.br
Neurosci Lett ; 439(2): 170-2, 2008 Jul 11.
Article en En | MEDLINE | ID: mdl-18524484
The purpose of the present work was to investigate the pharmacological action of a calcium channel-blocking toxin from the venom of the spider Phonetic nigriventer, Tx3-4 on calcium channels coupled to exocytosis of synaptic vesicles. Tx3-4 blocked KCl-induced exocytosis of synaptic vesicles with an IC50 of 1.1 nM. To investigate whether the target of Tx3-4 overlaps with known calcium channels that mediate calcium entry and exocytosis, we used omega-toxins that interact selectively with neuronal calcium channels. The results indicate that the main population of voltage-sensitive calcium channels altered by Tx3-4 is P/Q calcium channels. In conclusion, Tx3-4 is a potent inhibitor of calcium channels involved in the KCl-induced exocytosis of synaptic vesicles in brain cortical synaptosomes.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Venenos de Araña / Canales de Calcio / Exocitosis / Neurotoxinas Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Neurosci Lett Año: 2008 Tipo del documento: Article País de afiliación: Brasil

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Venenos de Araña / Canales de Calcio / Exocitosis / Neurotoxinas Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Neurosci Lett Año: 2008 Tipo del documento: Article País de afiliación: Brasil