[Determination of 1, 2, 3, 4, 6-penta-O-galloyl-D-glucose in forty four kinds of Chinese traditional medicines by HPLC].
Zhongguo Zhong Yao Za Zhi
; 33(6): 656-9, 2008 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-18590194
ABSTRACT
OBJECTIVE:
To determine 1, 2, 3, 4, 6-penta-O-galloyl-D-glucose (PGG) in forty four kinds of Chinese traditional medicines by HPLC.METHOD:
The PGG was extracted with a solvent consisting of ethanol and water (5050) in ultrasonic bath at 50-60 degrees C for 1 hour. A Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was used for the separation and analysis of PGG with a mobile phase consisting of acetonitrile and 2. 5 % acetic acid solution (1882). The wavelength of detection was at 280 nm, and flow rate was set at 1.0 mL min(-1).RESULT:
The calibration curve for PGG is linear over the range of 1-150 microg mL(-1) (r =0.9994), and PGG was found in seventeen kinds of Chinese traditional medicines, such as gall tree peony bark, red peony root, white peony root, and so on.CONCLUSION:
The contents of PGG were determined in forty four kinds of Chinese traditional medicines by a rapid and precision HPLC method.
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Bases de datos:
MEDLINE
Asunto principal:
Medicamentos Herbarios Chinos
/
Taninos Hidrolizables
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2008
Tipo del documento:
Article
País de afiliación:
China