[Determination of 1, 2, 3, 4, 6-penta-O-galloyl-D-glucose in forty four kinds of Chinese traditional medicines by HPLC].

ABSTRACT

OBJECTIVE: To determine 1, 2, 3, 4, 6-penta-O-galloyl-D-glucose (PGG) in forty four kinds of Chinese traditional medicines by HPLC. METHOD: The PGG was extracted with a solvent consisting of ethanol and water (5050) in ultrasonic bath at 50-60 degrees C for 1 hour. A Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was used for the separation and analysis of PGG with a mobile phase consisting of acetonitrile and 2. 5 % acetic acid solution (1882). The wavelength of detection was at 280 nm, and flow rate was set at 1.0 mL min(-1). RESULT: The calibration curve for PGG is linear over the range of 1-150 microg mL(-1) (r =0.9994), and PGG was found in seventeen kinds of Chinese traditional medicines, such as gall tree peony bark, red peony root, white peony root, and so on. CONCLUSION: The contents of PGG were determined in forty four kinds of Chinese traditional medicines by a rapid and precision HPLC method.