Genetic, genomic and physiological state studies on single-needle bio-electrosprayed human cells.
Analyst
; 133(10): 1347-51, 2008 Oct.
Article
en En
| MEDLINE
| ID: mdl-18810280
ABSTRACT
Bio-electrospraying, a non-contact jet-based direct cell engineering approach, was recently pioneered and demonstrated for handling a wide range of primary living cells. In those studies, post-treated cells were biologically assessed in comparison to several controls by way of flow cytometry. Although flow cytometry accurately assesses those viable populations of cells, subtle effects at a sub-cellular level could have been missed. Therefore, in the present study we demonstrate metaphase chromosome breakage studies carried out on single-needle bio-electrosprayed human T-lymphocytes, which are compared with several controls. The results indicate that post-treated T-lymphocytes do not demonstrate any increase in chromosome damage in comparison to control cells. These studies further validate bio-electrospraying as a technique with potential for clinical utility.
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1
Bases de datos:
MEDLINE
Asunto principal:
Linfocitos T
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Rotura Cromosómica
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Ingeniería de Tejidos
Límite:
Humans
Idioma:
En
Revista:
Analyst
Año:
2008
Tipo del documento:
Article