Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing.
Nat Methods
; 6(1): 67-9, 2009 Jan.
Article
en En
| MEDLINE
| ID: mdl-19079253
ABSTRACT
Our variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected approximately 98% of differences (including large insertion-deletions) between pairs of strains from three species while calling no false positives. VAAL also pinpointed a single mutation between Vibrio cholerae genomes, identifying an antibiotic's site of action by identifying sequence differences between drug-sensitive strains and drug-resistant derivatives.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Polimorfismo Genético
/
ADN Bacteriano
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos