Role of Ca2+ in t-PA production by human embryonic lung diploid fibroblast, IMR-90 cells, stimulated by proteose peptone.
Cell Signal
; 3(2): 99-105, 1991.
Article
en En
| MEDLINE
| ID: mdl-1908695
ABSTRACT
Proteose peptone (p.peptone) remarkably induced tissue plasminogen activator (t-PA) activity in the conditioned medium of confluently cultured human embryonic lung diploid fibroblast, IMR-90 cells, in a dose-dependent manner. t-PA activity correlated well with the amount of t-PA antigen found in the conditioned medium of IMR-90 cells stimulated by p.peptone. t-PA production by IMR-90 cells stimulated by p.peptone was dependent on extracellular Ca2+ concentration and maximum t-PA production required approximately 3.6 mM extracellular Ca2+. Conversely, elimination of Ca2+ from the culture medium by EGTA, Ca2+ chelate agent, strongly inhibited t-PA production induced by p.peptone. t-PA production induced by p.peptone was inhibited in a dose-dependent manner by Verapamil, which inhibits Ca2+ uptake through the slow channels and also by W-7, an inhibitor of calmodulin. These results suggested that influx of extracellular Ca2+ into IMR-90 cells was caused by p.peptone and induced t-PA production by the cells.
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Bases de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Peptonas
/
Caseínas
/
Calcio
/
Activador de Tejido Plasminógeno
Límite:
Humans
Idioma:
En
Revista:
Cell Signal
Año:
1991
Tipo del documento:
Article
País de afiliación:
Japón