Serum protein profiling by solid phase extraction and mass spectrometry: a future diagnostics tool?
Proteomics
; 9(6): 1428-41, 2009 Mar.
Article
en En
| MEDLINE
| ID: mdl-19235169
ABSTRACT
Serum protein profiling by MS is a promising method for early detection of disease. Important characteristics for serum protein profiling are preanalytical factors, analytical reproducibility and high throughput. Problems related to preanalytical factors can be overcome by using standardized and rigorous sample collection and sample handling protocols. The sensitivity of the MS analysis relies on the quality of the sample; consequently, the blood sample preparation step is crucial to obtain pure and concentrated samples and enrichment of the proteins and peptides of interest. This review focuses on the serum sample preparation step prior to protein profiling by MALDI MS analysis, with particular focus on various SPE methods. The application of SPE techniques with different chromatographic properties such as RP, ion exchange, or affinity binding to isolate specific subsets of molecules (subproteomes) is advantageous for increasing resolution and sensitivity in the subsequent MS analysis. In addition, several of the SPE sample preparation methods are simple and scalable and have proven easy to automate for higher reproducibility and throughput, which is important in a clinical proteomics setting.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Proteínas Sanguíneas
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Química Clínica
/
Extracción en Fase Sólida
Tipo de estudio:
Diagnostic_studies
/
Guideline
/
Screening_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Proteomics
Asunto de la revista:
BIOQUIMICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Dinamarca