Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of prednisolone and dipyridamole in human plasma and its application in a pharmacokinetic study.
J Pharm Biomed Anal
; 49(5): 1241-9, 2009 Jul 12.
Article
en En
| MEDLINE
| ID: mdl-19304431
ABSTRACT
We have developed and validated an accurate, sensitive, and robust LC-MS/MS method that determines the concentration of CRx-102 (the combination of prednisolone and dipyridamole) in human plasma. In this method, prednisolone, dipyridamole, and the combined internal standards (IS) prednisolone-d(6) (IS for prednisolone) and dipyridamole-d(20) (IS for dipyridamole) were extracted from 100 microL human EDTA plasma using methylbutyl ether. Calibration curves were linear over a concentration range of 0.4-200 ng/mL for prednisolone and 5-3000 ng/mL for dipyridamole. The analytes were quantitatively determined using tandem mass spectrometry operated in positive electrospray ionization in a multiple reaction monitoring (MRM) mode. This validated method has been used successfully in clinical pharmacokinetic studies of CRx-102 in healthy volunteers.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Prednisolona
/
Cromatografía Liquida
/
Dipiridamol
/
Espectrometría de Masas en Tándem
Tipo de estudio:
Diagnostic_studies
/
Guideline
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos