RNA-directed DNA methylation requires an AGO4-interacting member of the SPT5 elongation factor family.
EMBO Rep
; 10(6): 649-54, 2009 Jun.
Article
en En
| MEDLINE
| ID: mdl-19343051
ABSTRACT
Recent studies have identified a conserved WG/GW-containing motif, known as the Argonaute (AGO) hook, which is involved in the recruitment of AGOs to distinct components of the eukaryotic RNA silencing pathways. By using this motif as a model to detect new components in plant RNA silencing pathways, we identified SPT5-like, a plant-specific AGO4-interacting member of the nuclear SPT5 (Suppressor of Ty insertion 5) RNA polymerase (RNAP) elongation factor family that is characterized by the presence of a carboxy-terminal extension with more than 40 WG/GW motifs. Knockout SPT5-like mutants show a decrease in the accumulation of several 24-nt RNAs and hypomethylation at different loci revealing an implication in RNA-directed DNA methylation (RdDM). Here, we propose that SPT5-like emerged in plants as a facultative RNAP elongation factor. Its plant-specific origin and role in RdDM might reflect functional interactions with plant-specific RNA Pols required for RdDM.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas Cromosómicas no Histona
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Arabidopsis
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ARN de Planta
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Metilación de ADN
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Proteínas de Arabidopsis
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Factores de Elongación Transcripcional
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
EMBO Rep
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia