Micromachined hydrogel stamper for soft printing of biomolecules with adjustable feature dimensions.
Anal Chem
; 81(11): 4551-4, 2009 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-19405482
ABSTRACT
We report on the development of a hydrogel stamper with built-in reservoirs for soft-printing of biomolecules with adjustable feature dimensions. The stamper consists of potassium hydroxide (KOH)-etched silicon cavities onto which biomolecule-soaked low cross-linked density hydrogel with large pores and low mechanical strength is loaded. Application of weight to the top surface allows for a controllable protrusion of hydrogel from the opposite nozzles. Such protrusion combined with a suitable spacer between the stamper and a substrate provides a means for printing features with dimensions depending on the applied weight. Utilizing the above method, we successfully stamped bovine serum albumin conjugated with fluorescein isothiocyanate (BSA-FITC) model proteins on hydrophilic silicon substrates with a feature dimension ratio of 201 using a single stamper.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Microtecnología
/
Geles
Límite:
Animals
Idioma:
En
Revista:
Anal Chem
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos