Dynein and mast/orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics.
J Cell Sci
; 122(Pt 14): 2543-53, 2009 Jul 15.
Article
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| MEDLINE
| ID: mdl-19549687
ABSTRACT
Establishment and maintenance of the mitotic spindle requires the balanced activity of microtubule-associated proteins and motors. In this study we have addressed how the microtubule plus-end tracking protein mast/orbit/CLASP and cytoplasmic dynein regulate this process in Drosophila melanogaster embryos and S2 cells. We show that mast accumulates at kinetochores early in mitosis, which is followed by a poleward streaming upon microtubule attachment. This leads to a reduction of mast levels at kinetochores during metaphase and anaphase that depends largely on the microtubule minus end-directed motor cytoplasmic dynein. Surprisingly, we also found that co-depletion of dynein rescues spindle bipolarity in mast-depleted cells, while restoring normal microtubule poleward flux. Our results suggest that mast and dynein have antagonistic roles in the local regulation of microtubule plus-end dynamics at kinetochores, which are important for the maintenance of spindle bipolarity and normal spindle length.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Transducción de Señal
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Dineínas
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Cinetocoros
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Proteínas de Drosophila
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Drosophila melanogaster
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Proteínas Asociadas a Microtúbulos
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Microtúbulos
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Mitosis
Límite:
Animals
Idioma:
En
Revista:
J Cell Sci
Año:
2009
Tipo del documento:
Article
País de afiliación:
Portugal