Probing the membrane potential of living cells by dielectric spectroscopy.
Eur Biophys J
; 38(8): 1049-59, 2009 Oct.
Article
en En
| MEDLINE
| ID: mdl-19579022
ABSTRACT
In this paper we demonstrate a quantitative way to measure the membrane potential of live cells by dielectric spectroscopy. We also show that the values of the membrane potential obtained using our technique are in good agreement with those obtained using traditional methods-voltage-sensitive dyes. The membrane potential is determined by fitting the experimental dielectric dispersion curves with the dispersion curves obtained from a theoretical model. Variations in the membrane potential were induced by modifying the concentration of potassium chloride in the solution of the cell suspension in the presence of valinomycin. For exemplification of the method, E. coli was chosen for our experiments.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Análisis Espectral
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Técnicas Citológicas
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Escherichia coli
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Potenciales de la Membrana
Idioma:
En
Revista:
Eur Biophys J
Asunto de la revista:
BIOFISICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos