Gene expression down-regulation in CD90+ prostate tumor-associated stromal cells involves potential organ-specific genes.
BMC Cancer
; 9: 317, 2009 Sep 08.
Article
en En
| MEDLINE
| ID: mdl-19737398
ABSTRACT
BACKGROUND:
The prostate stroma is a key mediator of epithelial differentiation and development, and potentially plays a role in the initiation and progression of prostate cancer. The tumor-associated stroma is marked by increased expression of CD90/THY1. Isolation and characterization of these stromal cells could provide valuable insight into the biology of the tumor microenvironment.METHODS:
Prostate CD90+ stromal fibromuscular cells from tumor specimens were isolated by cell-sorting and analyzed by DNA microarray. Dataset analysis was used to compare gene expression between histologically normal and tumor-associated stromal cells. For comparison, stromal cells were also isolated and analyzed from the urinary bladder.RESULTS:
The tumor-associated stromal cells were found to have decreased expression of genes involved in smooth muscle differentiation, and those detected in prostate but not bladder. Other differential expression between the stromal cell types included that of the CXC-chemokine genes.CONCLUSION:
CD90+ prostate tumor-associated stromal cells differed from their normal counterpart in expression of multiple genes, some of which are potentially involved in organ development.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Próstata
/
Neoplasias de la Próstata
/
Antígenos CD
/
Regulación hacia Abajo
/
Células del Estroma
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
BMC Cancer
Asunto de la revista:
NEOPLASIAS
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos