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Dual role for the methyltransferase G9a in the maintenance of beta-globin gene transcription in adult erythroid cells.
Chaturvedi, Chandra-Prakash; Hosey, Alison M; Palii, Carmen; Perez-Iratxeta, Carolina; Nakatani, Yoshihiro; Ranish, Jeffrey A; Dilworth, F Jeffrey; Brand, Marjorie.
Afiliación
  • Chaturvedi CP; The Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON Canada.
Proc Natl Acad Sci U S A ; 106(43): 18303-8, 2009 Oct 27.
Article en En | MEDLINE | ID: mdl-19822740
Using a proteomics screen, we have identified the methyltransferase G9a as an interacting partner of the hematopoietic activator NF-E2. We show that G9a is recruited to the beta-globin locus in a NF-E2-dependent manner and spreads over the entire locus. While G9a is often regarded as a corepressor, knocking down this protein in differentiating adult erythroid cells leads to repression of the adult beta(maj) globin gene and aberrant reactivation of the embryonic beta-like globin gene E(y). While in adult cells G9a maintains E(y) in a repressed state via dimethylation of histone H3 at lysines 9 and 27, it activates beta(maj) transcription in a methyltransferase-independent manner. Interestingly, the demethylase UTX is recruited to the beta(maj) (but not the E(y)) promoter where it antagonizes G9a-dependent H3K27 dimethylation. Collectively, these results reveal a dual role for G9a in maintaining proper expression (both repression and activation) of the beta-globin genes in differentiating adult erythroid cells.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Transcripción Genética / Envejecimiento / N-Metiltransferasa de Histona-Lisina / Células Eritroides / Globinas beta Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2009 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Transcripción Genética / Envejecimiento / N-Metiltransferasa de Histona-Lisina / Células Eritroides / Globinas beta Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2009 Tipo del documento: Article