Mechanism of tumor cell-induced T-cell apoptosis mediated by galectin-1.
Immunol Lett
; 127(2): 108-18, 2010 Jan 04.
Article
en En
| MEDLINE
| ID: mdl-19874850
ABSTRACT
Galectin-1 (Gal-1) has been implicated in tumor progression partly via the induction of T-cell apoptosis. However the mechanism of Gal-1 induced T-cell death was mostly studied using recombinant, soluble Gal-1 producing controversial results. To explore the true mechanism of Gal-1 and hence tumor cell-induced T-cell death, we applied co-cultures of tumor cells and T-cells thus avoiding artificial circumstances generated using recombinant protein. T-cells died when co-cultured with Gal-1-expressing but survived with Gal-1 non-expressing tumor cells. Removing tumor cell surface Gal-1 or knocking down Gal-1 expression resulted in diminution of T-cell apoptosis. Gal-1 transgenic or soluble Gal-1 treated HeLa cells became cytotoxic. Stimulation of apoptosis required interaction between the tumor and T-cells, presence of p56lck and ZAP70, decrease of mitochondrial membrane potential and caspase activation. Hence tumor cell-derived Gal-1 might efficiently contribute to tumor self-defense. Moreover this system resolves the discrepancies obtained using recombinant Gal-1 in T-cell apoptosis studies.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Linfocitos T
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Apoptosis
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Galectina 1
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Mitocondrias
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Proteínas de Neoplasias
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Neoplasias
Límite:
Humans
Idioma:
En
Revista:
Immunol Lett
Año:
2010
Tipo del documento:
Article
País de afiliación:
Hungria