[Micronucleus rate and chromosome aberration of mouse embryo liver induced by formaldehyde].

ABSTRACT

OBJECTIVE: To study the micronucleus rate and chromosome aberration of mouse embryo liver induced by formaldehyde. METHODS: The pregnant mice (been pregnant for 13 days) were divided into 5 groups randomly Formaldehyde was given as intraperitoneal injection (0.00, 0.20, 2.00, 20.00 mg/kg) for exposure group and Cyclophosphamide (30 mg/kg) for positive control group. When the 14th days (i.e After exposed in formaldehyde 18 hours), escapes the cervical vertebra cervical killed off pregnant mouse, peels on both sides of the uterus, takes an embryo respectively each side, beheads and emits the circumference blood, takes out the embryo liver, performs micronucleus experiment and chromosome aberration experiment by use of embryo liver, validate embryo liver blood micronucleus rate and the chromosome distortion factor. RESULTS: The micronucleus rate and chromosome aberration (mainly for chromosome breakage, polyploid) rate of embryonic liver showed significant differences in formaldehyde exposure group (2.00, 20.00 mg/kg) compared with the control group (P < 0.01). CONCLUSION: The micronucleus rate and chromosome aberration of mouse embryos liver induced by formaldehyde.