New method for the study of Amaryllidaceae alkaloid biosynthesis using biotransformation of deuterium-labeled precursor in tissue cultures.
Acta Biochim Pol
; 57(1): 75-82, 2010.
Article
en En
| MEDLINE
| ID: mdl-20066175
ABSTRACT
Biotransformation of deuterated-4'-O-methylnorbelladine into alkaloids galanthamine and lycorine in tissue cultures of Leucojum aestivum was demonstrated using HPLC coupled to mass spectrometry. GC-MS screening was also carried to investigate other native and deuterated alkaloids. A total of six labeled alkaloids were identified indicating that 4'-O-methyl-d(3)-norbelladine is incorporated into three different groups of Amaryllidaceae alkaloids that are biosynthesized by three modes of intramolecular oxidative phenol coupling.
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Bases de datos:
MEDLINE
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Liliaceae
/
Alcaloides de Amaryllidaceae
/
Cromatografía de Gases y Espectrometría de Masas
Idioma:
En
Revista:
Acta Biochim Pol
Año:
2010
Tipo del documento:
Article
País de afiliación:
Francia