Diazepam decreases leukocyte-endothelium interactions in situ.
Immunopharmacol Immunotoxicol
; 32(3): 402-9, 2010 Sep.
Article
en En
| MEDLINE
| ID: mdl-20095803
ABSTRACT
High doses of diazepam reduce the inflammatory paw edema in rats. This effect was attributed to an action of diazepam on the Translocator Protein (TSPO). We evaluated the effects of diazepam (10 mg/kg, intraperitoneally) on leukocyte rolling and migration. In carrageenan-induced acute inflammation, diazepam decreased the interaction of leukocytes with endothelial cells (rolling) and the number of leukocytes in the mesentery (migration). RU486 (antagonist of glucocorticoid receptors) reduced the effects of diazepam on leukocyte rolling and migration, suggesting a participation of endogenous corticosteroids. We also showed that the effects of diazepam on leukocyte-endothelium interactions are mediated by nitric oxide (NO), since prior treatment with l-arginine (precursor of NO) partially precludes the inhibitory effects of diazepam; conversely, pretreatment with L-NAME (false substrate of the NO synthase) somewhat potentiates the effects of diazepam. The pathways that underlie the effects of diazepam remain to be further elucidated, but we believe that both local and systemic mechanisms may overlap to explain the influence of diazepam on leukocyte-endothelium interactions.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Psicotrópicos
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Endotelio Vascular
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Rodamiento de Leucocito
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Diazepam
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Inflamación
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Leucocitos
Límite:
Animals
Idioma:
En
Revista:
Immunopharmacol Immunotoxicol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
FARMACOLOGIA
/
TOXICOLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Brasil