Acid-sensing ion channels in acidosis-induced injury of human brain neurons.
J Cereb Blood Flow Metab
; 30(6): 1247-60, 2010 Jun.
Article
en En
| MEDLINE
| ID: mdl-20216553
ABSTRACT
Acidosis is a common feature of the human brain during ischemic stroke and is known to cause neuronal injury. However, the mechanism underlying acidosis-mediated injury of the human brain remains elusive. We show that a decrease in the extracellular pH evoked inward currents characteristic of acid-sensing ion channels (ASICs) and increased intracellular Ca(2+) in cultured human cortical neurons. Acid-sensing ion channels in human cortical neurons show electrophysiological and pharmacological properties distinct from those in neurons of the rodent brain. Reverse transcriptase-PCR and western blot detected a high level of the ASIC1a subunit with little or no expression of other ASIC subunits. Treatment of human cortical neurons with acidic solution induced substantial cell injury, which was attenuated by the ASIC1a blockade. Thus, functional homomeric ASIC1a channels are predominantly expressed in neurons from the human brain. Activation of these channels has an important role in acidosis-mediated injury of human brain neurons.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Acidosis
/
Canales de Sodio
/
Corteza Cerebral
/
Regulación de la Expresión Génica
/
Proteínas del Tejido Nervioso
/
Neuronas
Límite:
Adult
/
Aged
/
Animals
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Cereb Blood Flow Metab
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos