On the mechanism of oscillations in neutrophils.
Biophys Chem
; 148(1-3): 82-92, 2010 May.
Article
en En
| MEDLINE
| ID: mdl-20227815
ABSTRACT
We have investigated the regulation of the oscillatory generation of H(2)O(2) and oscillations in shape and size in neutrophils in suspension. The oscillations are independent of cell density and hence do not represent a collective phenomena. Furthermore, the oscillations are independent of the external glucose concentration and the oscillations in H(2)O(2) production are 180 degrees out of phase with the oscillations in NAD(P)H. Cytochalasin B blocked the oscillations in shape and size whereas it increased the period of the oscillations in H(2)O(2) production. 1- and 2-butanol also blocked the oscillations in shape and size, but only 1-butanol inhibited the oscillations in H(2)O(2) production. We conjecture that the oscillations are likely to be due to feedback regulations in the signal transduction cascade involving phosphoinositide 3-kinases (PI3K). We have tested this using a simple mathematical model, which explains most of our experimental observations.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Modelos Biológicos
/
Neutrófilos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Biophys Chem
Año:
2010
Tipo del documento:
Article
País de afiliación:
Dinamarca