Fluorescence quenched quinone methide based activity probes--a cautionary tale.
Org Biomol Chem
; 8(7): 1610-8, 2010 Apr 07.
Article
en En
| MEDLINE
| ID: mdl-20237672
ABSTRACT
A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Carbamatos
/
Arabidopsis
/
Indolquinonas
/
Carboxilesterasa
/
Colorantes Fluorescentes
Idioma:
En
Revista:
Org Biomol Chem
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2010
Tipo del documento:
Article