Detection of prostate-specific antigen with a paired surface plasma wave biosensor.
Anal Chem
; 82(9): 3714-8, 2010 May 01.
Article
en En
| MEDLINE
| ID: mdl-20373787
ABSTRACT
In this study, we demonstrated that an amplitude-sensitive paired surface plasma wave biosensor (PSPWB) is capable of real-time detection of prostate-specific antigen (PSA) in diluted human serum without labeling. Experimentally, the detection limit of PSPWB was 8.4 x 10(-9) refractive index unit (RIU) and the PSPWB could measure PSA in a phosphate buffered saline solution from 10 fg/mL ( approximately 300 aM) to 100 pg/mL ( approximately 3 pM) successfully, with demonstration of a linear relationship between PSA concentrations and surface plasmon resonance (SPR) signals. Therefore, results were obtained over a wide dynamic range 5 orders of magnitude for analyte concentration. In addition, the PSPWB successfully detected PSA in diluted human serum as well. These experimental results indicate that the PSPWB is capable of detection with high sensitivity over a wide range by using SPR-based biosensors and has a capability of detecting biological analytes in clinical sample without complicated operating procedures.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Antígeno Prostático Específico
/
Resonancia por Plasmón de Superficie
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
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Male
Idioma:
En
Revista:
Anal Chem
Año:
2010
Tipo del documento:
Article