Molecular assay for screening and quantifying DNA in biological evidence: the modified Q-TAT assay.
J Forensic Sci
; 55(4): 1050-7, 2010 Jul.
Article
en En
| MEDLINE
| ID: mdl-20384933
ABSTRACT
A method is described for the quantitation of total human and male DNA. Q-TAT utilizes end-point, multiplex polymerase chain reaction (PCR) amplification of the amelogenin and SRY loci to quantify DNA and incorporates a cloned nonhuman template to detect PCR inhibition. Standard curves of fluorescence from amelogenin or SRY amplicons were generated from amplification of known amounts of NIST traceable SRM-female or SRM-male DNA. Curves showed good linearity up to 500 pg of SRM-template (R(2) > 0.99) and reliably estimated total and male DNA content in casework samples. The nonhuman pRL(null) template included in each PCR was a sensitive indicator of known PCR inhibitors including EDTA, hemin, blue denim dye, and humic acid. Finally, the SRY amplicon was a sensitive indicator of male DNA and, in mixtures, could reliably estimate male DNA present in an excess of female DNA. The Q-TAT multiplex is a reliable quantitation method for forensic DNA typing.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Dermatoglifia del ADN
/
Genes sry
/
Cromosomas Humanos Y
/
Luciferasas de Renilla
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Forensic Sci
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos