Temperature sensing using fluorescent nanothermometers.
ACS Nano
; 4(6): 3254-8, 2010 Jun 22.
Article
en En
| MEDLINE
| ID: mdl-20441184
ABSTRACT
Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF(4)Er(3+),Yb(3+) nanoparticles, where the intensity ratio of the green fluorescence bands of the Er(3+) dopant ions ((2)H(11/2) --> (4)I(15/2) and (4)S(3/2) --> (4)I(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF(4)Er(3+),Yb(3+) nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.
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1
Bases de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
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Termómetros
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Nanotecnología
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
ACS Nano
Año:
2010
Tipo del documento:
Article