ChIP on Chip: surprising results are often artifacts.
BMC Genomics
; 11: 414, 2010 Jul 05.
Article
en En
| MEDLINE
| ID: mdl-20602746
ABSTRACT
BACKGROUND:
The method of chromatin immunoprecipitation combined with microarrays (ChIP-Chip) is a powerful tool for genome-wide analysis of protein binding. However, a high background signal is a common phenomenon.RESULTS:
Reinvestigation of the chromatin immunoprecipitation procedure led us to discover four causes of highbackground:
i) non-unique sequences, ii) incomplete reversion of crosslinks, iii) retention of protein in spin-columns and iv) insufficient RNase treatment. The chromatin immunoprecipitation method was modified and applied to analyze genome-wide binding of SeqA and sigma(32) in Escherichia coli.CONCLUSIONS:
False positive findings originating from these shortcomings of the method could explain surprising and contradictory findings in published ChIP-Chip studies. We present a modified chromatin immunoprecipitation method greatly reducing the background signal.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Artefactos
/
Análisis por Matrices de Proteínas
/
Inmunoprecipitación de Cromatina
Idioma:
En
Revista:
BMC Genomics
Asunto de la revista:
GENETICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Noruega